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The objective was to evaluate the resistance of soybean lines to rust disease. The research was conducted in the greenhouse of Indonesian Legume and Tuber Crops Research Institute, from March to June Design was randomized completely block design with three replications. Materials were baku mutu kualitas udara ambien provinsi jawa tengah soybean lines and five check varieties. Inoculation was performed by spraying the spore suspension on the first leaves at Observations rust disease resistance based on the system of International Working Group on Soybean Rust. Results showed that there was no soybean line identified as resistant line. The three check varieties Wilis, Tanggamus and Soma drug used for were moderately resistant, while two check varieties Grobogan and Argomulyo were moderately susceptible. B Keywords soybean lines, resistance, rust disease.

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